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The activation phase, the second step in the canonical pathway, is driven by diverse stimuli such as K + efflux, ROS overproduction, lysosomal rupture, mitochondrial DNA (mtDNA) leakage, and organelle dysfunction (e.g., mitochondria, Golgi apparatus, endoplasmic reticulum) (Santos et al., 2018), which can be recognized by caspase-11 (the mouse homolog of human caspase-4/5)

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Therefore, this advanced biogenic compound serves as a critical baseline for targeted soft tissue repair research globally

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