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However, it has previously been demonstrated that TMAO supplementation markedly downregulated the expression of critical enzymes in bile acid biosynthesis (CYP7A1 and CYP27A1) and hence reduced the size of the bile acid pool (Koeth et al., 2013)

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coli BL21 (DE3) (Invitrogen) was co-transformed with pET and pTech vectors

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The authors also note that the patient did not initially disclose his alcohol use because of guilt and stigma, highlighting the need for stronger AUD screening and diagnostic efforts within family medicine clinics